MDPI’s Post

🔬 Research Alert! published in #DrugDevIndPharm. 2023, VOL. 49, NO. 2, 149–158 🌟Oral formulation of Prussian blue with improved efficacy for prophylactic use against thallium🌟 This research article focuses on the development and evaluation of a formulation for the removal of thallium (Tl) in rats. The study involved the preparation and optimization of pH modifier granules, as well as the evaluation of the final formulation's stability and efficacy in removing Tl from the blood. The results showed that the developed formulation (FF4) had high stability and binding ability with Tl, leading to a significant reduction in blood Tl levels in rats. 📖 Read the full article here: https://lnkd.in/gNVusNM7

Recent advances on antimicrobial peptide and polysaccharide hydrogels

Collagen is a major constituent of the extracellular matrix and the predominant protein found in animals, making up approximately 30% of all protein mass. Collagens are characterized by possessing at least one ‘domain’ of triple helix structure. At least 28 different types of collagens have been identified, with Type I collagen being the most prevalent form, particularly in ligaments, tendons, skin, and bone tissue. The biophysical properties of mature, ‘insoluble collagen’ allow it to withstand high tensile forces as well as being resistant to stretching. These are essential properties that enable the locomotion of organisms. Collagen also exhibits key biochemical properties, being involved in cell growth, proliferation, and differentiation. This 'biochemical' form of collagen is typically recently synthesized and, due to minimal crosslinking, can be readily solubilized via cold acid or enzymatic digestion. This is commonly referred to as ‘Soluble Collagen’. Aged collagen undergoes extensive cross-linking and therefore remains ‘insoluble’ under cold acid or enzymatic digestion. We, BTL Biotechno Labs Pvt. Ltd., an authorized distributor of Biocolor Life Science Assays, take pride in offering their user-friendly quantification of soluble collagens (Sircol 2.0 kit) to the Indian scientific community working on ECM and cell signaling/cell cycle research at competitive prices. For more details, please connect with us at info@biotechnolabs.com. Biocolor Ltd David Steele Martyna Bailie #lifesciences #researchanddevelopment #biotechnologyindustry #pharmaceuticals #protein #collagen #collagentype1 #solublecollagen #cellsignaling #extracellularmatrix #enzymaticdigestion #solublecollagenassaykit #biocolorlifescienceassay #btlbiotechnolabspvtltd  

Collagen is a major constituent of the extracellular matrix and the predominant protein found in animals, making up approximately 30% of all protein mass. Collagens are characterized by possessing at least one ‘domain’ of triple helix structure. At least 28 different types of collagens have been identified, with Type I collagen being the most prevalent form, particularly in ligaments, tendons, skin, and bone tissue. The biophysical properties of mature, ‘insoluble collagen’ allow it to withstand high tensile forces as well as being resistant to stretching. These are essential properties that enable the locomotion of organisms. Collagen also exhibits key biochemical properties, being involved in cell growth, proliferation, and differentiation. This 'biochemical' form of collagen is typically recently synthesized and, due to minimal crosslinking, can be readily solubilized via cold acid or enzymatic digestion. This is commonly referred to as ‘Soluble Collagen’. Aged collagen undergoes extensive cross-linking and therefore remains ‘insoluble’ under cold acid or enzymatic digestion. We, BTL Biotechno Labs Pvt. Ltd., an authorized distributor of Biocolor Life Science Assays, take pride in offering their user-friendly quantification of soluble collagens (Sircol 2.0 kit) to the Indian scientific community working on ECM and cell signaling/cell cycle research at competitive prices. For more details, please connect with us at info@biotechnolabs.com. Biocolor Ltd David Steele Martyna Bailie #lifesciences #researchanddevelopment #biotechnologyindustry #pharmaceuticals #protein #collagen #collagentype1 #solublecollagen #cellsignaling #extracellularmatrix #enzymaticdigestion #solublecollagenassaykit #biocolorlifescienceassay #btlbiotechnolabspvtltd

𝗕𝗶𝗼𝘁𝗶𝗻 𝗕𝗶𝗻𝗱𝗶𝗻𝗴 𝗣𝗹𝗮𝘁𝗲 𝗠𝗮𝗿𝗸𝗲𝘁 𝟮𝟬𝟮𝟰-𝟮𝟬𝟯𝟭. 𝗚𝗹𝗼𝗯𝗮𝗹 𝗥𝗲𝘀𝗲𝗮𝗿𝗰𝗵 𝗥𝗲𝗽𝗼𝗿𝘁 The Biotin Binding Plate, also referred to as a streptavidin-coated plate, is a microplate treated with streptavidin, a protein that exhibits strong affinity towards biotin molecules. This modified surface facilitates the capture and fixation of biotinylated substances, such as proteins, nucleic acids, peptides, and diverse biomolecules, enabling applications like ELISA, protein interactions, and binding studies. The evolving trend in Biotin Binding Plates emphasizes enhanced binding capacity, minimized non-specific interactions, and compatibility with automation and high-throughput systems. There's a growing market demand for plates offering heightened sensitivity, reproducibility, and consistency in biotin binding, while also being adaptable to various assay formats and detection methods. The comprehensive report covers the industry's developmental chain, market standings within Hospital (High Bonding Surface, Low Binding Surface) and Laboratory segments (High Bonding Surface, Low Binding Surface), key players across developed and emerging markets, and a thorough analysis of leading-edge technologies, patents, applications, and market tendencies in Biotin Binding Plate technology. 𝗧𝗼 𝗞𝗻𝗼𝘄 𝗦𝗶𝘇𝗲 𝗮𝗻𝗱 𝗦𝗵𝗮𝗿𝗲 𝗼𝗳 𝗕𝗶𝗼𝘁𝗶𝗻 𝗕𝗶𝗻𝗱𝗶𝗻𝗴 𝗣𝗹𝗮𝘁𝗲 𝗠𝗮𝗿𝗸𝗲𝘁. 𝗥𝗲𝗾𝘂𝗲𝘀𝘁 𝗳𝗼𝗿 𝗥𝗲𝗽𝗼𝗿𝘁 𝗦𝗮𝗺𝗽𝗹𝗲 𝗣𝗗𝗙: https://lnkd.in/df3XNCKF *𝗕𝘆 𝗧𝘆𝗽𝗲: High Bonding Surface, Low Binding Surface *𝗕𝘆 𝗔𝗽𝗽𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻: Hospital, Laboratory, Other *𝗕𝘆 𝗥𝗲𝗴𝗶𝗼𝗻: North America, Europe, Asia-Pacific, South America, Middle East & Africa *𝗕𝘆 𝗞𝗲𝘆 𝗣𝗹𝗮𝘆𝗲𝗿𝘀: Thermo Fisher Scientific, Biomatter, PolyAn GmbH, Sigma-Aldrich, G-Biosciences, Biopanda Diagnostics, Monobind Inc., BioTeZ, MESO SCALE DIAGNOSTICS, LLC., BEAVER Biomedical Engineering Co.,Ltd #BiotinBindingPlate #StreptavidinSurface #MicroplateTechnology #AssayApplications #ELISAPlates #ProteinInteractions #HighThroughputAssays #BiomoleculeCapture #LabTech #BiotechInnovation

➡ In search of Inhaled #mRNA delivery Gaurav Sahay group has prepared Lipid Nanoparticles Consisting of following components: 1. Ionizable Lipid: DLin-MC-3-DMA: 2. Helper Lipid: DSPC: 3. PEG lipid: DMG-PEG 4. Cholesterol: (This was replaced by B-Sitosterol). ➡ The research concluded that replacement of Cholesterol with B-Sitosterol contributes to Extra-hepatic Delivery. ➡ The Findings are quite similar to Michael J Mitchell group at University of Pennsylvania In Which Cholesterol was replace with Bile acid Salts such as cholic acid, chenodeoxycholic acid, deoxycholic acid, or lithocholic acid. ➡ These Two Studies Confirm that Replacement of Cholesterol in LNP Formulation with similar alternatives leads to extra-hepatic delivery. Share your Thoughts? https://lnkd.in/e7T7mnyH

The Ora Controlled Adverse Environment (CAE®) Dry Eye Challenge model immerses study participants in controlled stress environments and uses visual tasks to minimize variability in response. The data obtained from the CAE® offers more precision in assessing the effectiveness of your medication, requiring fewer patients, fewer testing locations, and less time. Contact us at BD@oraclinical.com to tailor the CAE® model to your study needs! #ophthalmology #clinicalresearch #biotechnology

Review by Rodica Elena Ionescu丨Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions https://lnkd.in/gJad6mCw MDPI; Université de Technologie de Troyes #biosensors #biomarker #openaccess #Abstract Currently, several biosensors are reported to confirm the absence/presence of an abnormal level of specific human biomarkers in research laboratories. Unfortunately, public marketing and/or pharmacy accessibility are not yet possible for many bodily fluid biomarkers. The questions are numerous, starting from the preparation of the substrates, the wet/dry form of recognizing the (bio)ligands, the exposure time, and the choice of the running buffers. In this context, for the first time, the present overview summarizes the pre-functionalization of standard and nanostructured solid/flexible supports with cysteamine (Cys) and glutaraldehyde (GA) chemicals for robust protein immobilization and detection of biomarkers in body fluids (serum, saliva, and urine) using three transductions: piezoelectrical, electrochemical, and optical, respectively. Thus, the reader can easily access and compare step-by-step conjugate protocols published over the past 10 years. In conclusion, Cys/GA chemistry seems widely used for electrochemical sensing applications with different types of recorded signals, either current, potential, or impedance. On the other hand, piezoelectric detection via quartz crystal microbalance (QCM) and optical detection by surface plasmon resonance (LSPR)/surface-enhanced Raman spectroscopy (SERS) are ultrasensitive platforms and very good candidates for the miniaturization of medical devices in the near future.

Nanoform’s Lead Scientist, Dr. Tamás Solymosi, participated in this month’s @DDD feature on #Bioavailability Solutions 👉 https://lnkd.in/gjgCTdYy.  “It is powerful in helping formulators decrease the particle size of a pure drug substance to the very lower edge of the nano range, which might offer dose re­duction, elimination of food effect, and lower variability in pharmacokinetic pa­rameters. In certain projects, nanoforming by CESS technology has enabled an order of magnitude increases in bioavailability, or the complete elimination of food inter­action.” Read more about our best-in-class #nanotechnology here 👉 https://nanoform.com/en/   #drugdevelopment #drugformulation #nanomedicine #particleengineering

Using cyclodextrins to prevent aggregation in protein formulations has been known for some time, and we have already published a lot about this topic. This paper from Florian Johann, Steffen Wöll, and Henning Gieseler adds some special spice to the application as they investigate the potential of CDs in reducing aggregation of Antibody–Drug Conjugates with different payloads. The main takeaways of the study are: Hydroxypropylated (CDs) reduced agitation-induced aggregation in all tested ADCs without causing destabilization during incubation at 40 °C. Native CDs that are not surface active partially reduced agitation-induced aggregation in ADCs, suggesting the possibility of stabilizing mechanisms beyond mere competition at the air-liquid interface. ADC-specific formulation effects were observed, e.g., superior performance of the large HPγCD type in stabilizing a highly unstable ADC with a bulky fluorophore payload during incubation. https://lnkd.in/dAtJZBSb