Twist Bioscience’s Post

In this application note from Twist Bioscience, learn how scientists enhanced protein engineering with synthetic libraries that provide complete or near-complete site representation, and high throughput and quality saturation mutagenesis libraries ready for downstream validation. #AD https://ow.ly/qcPF50QUntK

In this application note from Twist Bioscience, learn how scientists enhanced protein engineering with synthetic libraries that provide complete or near-complete site representation, and high throughput and quality saturation mutagenesis libraries ready for downstream validation. #AD https://ow.ly/fGjL50RKvSY

Many protein engineering strategies for library screening do not fully or uniformly represent every possible codon, suffering from amplification bias and sequence-specific limitations. In this application note from Twist Bioscience, learn how scientists enhanced protein engineering with synthetic libraries that provide complete or near-complete site representation, and high throughput and quality saturation mutagenesis libraries ready for downstream validation. Download the application note here #AD: https://ow.ly/EWP650Rv6MT

🚀 Check out this fascinating article on improved protein engineering with a synthetic variant library!!! #proteinengineering #sitesaturationlibraries #thinkDNAthinkTwist #wemakeDNA #DBTL Saturation mutagenesis is a commonly used technique in protein engineering, but it suffers from limitations of traditional methods such as amplification biases and incomplete access to codon mutational space. Twist Bioscience's Site Saturation Variant Libraries (SSVLs) provide a synthetic alternative that overcomes these technical challenges. A recent study benchmarked the performance of a Twist SSVL against an error-prone PCR (epPCR) library using a glucose activation assay in yeast. The SSVL produced greater variant representation and simplified downstream validation steps by providing complete variant diversity. This research demonstrates the potential of SSVLs to improve protein engineering and directed evolution applications. Check out the full article to learn more!

In this application note from Twist Bioscience, learn how scientists enhanced protein engineering with synthetic libraries that provide complete or near-complete site representation, and high throughput and quality saturation mutagenesis libraries ready for downstream validation. Click the link below to learn more about how scientists enhanced G protein-coupled receptor engineering with SSVLs. #AD https://ow.ly/npfg50Rv7cH

In this application note from Twist Bioscience, learn how scientists enhanced protein engineering with synthetic libraries that provide complete or near-complete site representation, and high throughput and quality saturation mutagenesis libraries ready for downstream validation. Click the link below to learn more about how scientists enhanced G protein-coupled receptor engineering with SSVLs #AD: https://ow.ly/L5K750QUn4S

Site Saturation Variant Libraries (SSVLs) are synthetic libraries for protein engineering. SSVLs are designed alternatives to libraries generated by random error-prone PCR (epPCR), a conventional technique that suffers from amplification bias, incomplete variant representation, and premature stop codons. Scientists use SSVLs to overcome the limitations of epPCR for protein engineering and directed evolution experiments. Download this application note from Twist Bioscience to learn how scientists enhanced G protein-coupled receptor engineering with SSVLs. #AD https://ow.ly/UmLb50Rv700

Exploring the potential for self-healing: Investigating the boundaries of protein creation in a synthetic cell-free system

More than half of all proteins are glycosylated, many of them at multiple sites. Did you know that already? Profiling of these glycosylation-related protein isoforms involves techniques to separate, identify, and quantify individual isoforms of glycoproteins and glycopeptides that are present in a biological specimen. Bruker's solutions, specifically the enablement of high-throughput processing empowered by the glyco-PASEF on timsTOF mass spectrometers, offer a cutting-edge approach for glycoprotein and glycan profiling. Empowering scientists with GlycoScape™ software and glyco-PASEF technology to achieve unparalleled precision in glycoprotein analysis. Follow the link for a unique opportunity to learn directly from Klaus Lindpaintner, Vice President, Glycobiology Solutions at Bruker. Key takeaways: 👩🔬 Discover how glyco-PASEF enables the precise targeting of glycopeptides, enhancing our understanding of glycoproteomics and its impact on disease mechanisms. 👨🔬 Learn about challenges in glycoproteomics and innovative solutions like GlycoScape™, which streamlines workflows by providing real-time glycoprotein identification readouts directly from your timsTOF data. #Science #Glycoproteomics #CancerResearch #timsTOF #GlycoScape #GlycoPASEF #Webinar #OnDemand #genuine #sciencecommunication

Integrated DNA Technologies & Whitehead Scientific Biological extractions are essential for the collection of nucleic acids and removal of PCR inhibitors before quantitative PCR (qPCR) reactions are performed. In this technical note we will explore using Integrated DNA Technologies’ PrimeTime™ One-Step 4X Broad-Range Master Mix as an alternative to other costlier and more time-consuming extraction approaches. Here, we show how extraction-free amplification can efficiently amplify cell culture samples when compared to controls. Further, the data show similar performance across all four markers queried. Download the technical note ➡️ https://bit.ly/47g8eKG 🛒 Order your Oligos online today ➡️ https://lnkd.in/g4MXB-_X Contact us today at whitesci@whitesci.co.za to talk to a specialist! #whiteheadscientific #oligos #DNA #science #biotech